New Polarity Suppressors in Escherichia coli: Suppression and Messenger RNA Stability
AUTOR(ES)
Carter, Timothy
RESUMO
Two new polarity suppressors (Su27 and Su78) have been isolated in Escherichia coli. Both suppress polarity in the trp and lac operons, and neither shows codon specificity for suppression. Extreme polar mutants of the lac z gene that contain either Su27 or Su78 make mRNA from the entire operon; however, no active β-galactosidase is translated from the z-gene messenger, and the amount of distal messenger is greater than would be expected from corresponding enzyme activities. Puromycin treatment of lac+ strains mimics the effect of polar chain-termination mutations in destabilizing lac mRNA. This effect is completely reversed by Su27, and it is partially reversed by Su78. The results suggest that these suppressors act by stabilizing mRNA after premature termination of protein synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389570Documentos Relacionados
- UGA suppression by normal tRNA Trp in Escherichia coli: codon context effects.
- Messenger RNA recognition in Escherichia coli: a possible second site of interaction with 16S ribosomal RNA.
- Stability of β-Galactosidase Messenger Ribonucleic Acid in Escherichia coli
- Messenger RNA structure and gene regulation at the translational level in Escherichia coli: the case of threonine:tRNAThr ligase.
- Messenger Ribonucleic Acid Stability in Relaxed and Stringent Escherichia coli Starved for Methionine
