New fluorescent calcium indicators designed for cytosolic retention or measuring calcium near membranes.
AUTOR(ES)
Vorndran, C
RESUMO
A new family of fluorescent calcium indicators has been developed based on a new analog of BAPTA called FF6. This new BAPTA analog serves as a versatile synthetic intermediate for developing Ca2+ indicators targeted to specific intracellular environments. Two of these new Ca2+ indicators, fura-PE3 and fura-FFP18, are described in this report. Fura-PE3 is a zwitterionic indicator that resists the rapid leakage and compartmentalization seen with fura-2 and other polycarboxylate calcium indicators. In contrast to results obtained with fura-2, cells loaded with PE3 remain brightly loaded and responsive to changes in concentration of cytosolic free calcium for hours. Fura-FFP18 is an amphipathic indicator that to binds to liposomes and to cell membranes. Studies to be detailed later indicate that FFP18 functions as a near-membrane Ca2+ indicator and that calcium levels near the plasma membrane rise faster and higher than in the cytosol.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1236445Documentos Relacionados
- A novel micropipet method for measuring the bending modulus of vesicle membranes.
- Micropipette suction for measuring piconewton forces of adhesion and tether formation from neutrophil membranes.
- Two calcium currents in Neanthes arenaceodentatus egg cell membranes.
- High-affinity fluorescent peptide binding to I-Ad in lipid membranes.
- Intramolecular excimer kinetics of fluorescent dipyrenyl lipids: 1. DMPC/cholesterol membranes.
