Negative regulators of the PHO system in Saccharomyces cerevisiae: isolation and structural characterization of PHO85.
AUTOR(ES)
Uesono, Y
RESUMO
One of the negative regulators of the PHO system of Saccharomyces cerevisiae, PHO85, has been isolated by transformation and complementation of a pho85 strain. The complementing activity was delimited within a 1258 bp DNA segment and this region has been sequenced. The largest open reading frame found in this region can encode a protein of 302 amino acid residues. A pho85 mutant resulted from disruption of the chromosomal counterpart of the open reading frame described above. Therefore, we concluded that the gene we have cloned is PHO85. This result also indicates that PHO85 is nonessential. Northern analysis revealed that the size of the PHO85 message is 1.1 kb. No similarity was found between the putative amino acid sequences of two negative regulators, the PHO80 and PHO85 proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=339945Documentos Relacionados
- The TRP4 gene of Saccharomyces cerevisiae: isolation and structural analysis.
- Structure-function relationships of the yeast cyclin-dependent kinase Pho85.
- Protease B of Saccharomyces cerevisiae: Isolation and Regulation of the PRB1 Structural Gene
- Isolation, physical characterization and expression analysis of the Saccharomyces cerevisiae positive regulatory gene PHO4.
- Cell Wall β-(1,6)-Glucan of Saccharomyces cerevisiae: STRUCTURAL CHARACTERIZATION AND IN SITU SYNTHESIS*S⃞