NahW, a Novel, Inducible Salicylate Hydroxylase Involved in Mineralization of Naphthalene by Pseudomonas stutzeri AN10

AUTOR(ES)

Bosch, Rafael

FONTE

American Society for Microbiology

RESUMO

Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated outside but in close proximity to this transcriptional unit. The nahG and nahW genes of P. stutzeri AN10 are induced and expressed upon incubation with salicylate, and the enzymes that are encoded, NahG and NahW, are involved in naphthalene and salicylate metabolism. Both genes, nahG and nahW, have been cloned in Escherichia coli JM109. The overexpression of these genes yields peptides with apparent molecular masses of 46 kDa (NahG) and 43 kDa (NahW), respectively. Both enzymes exhibit broad substrate specificities and metabolize salicylate, methylsalicylates, and chlorosalicylates. However, the relative rates by which the substituted analogs are transformed differ considerably.

Documentos Relacionados

• Salicylate formation from naphthalene by Pseudomonas aeruginosa.
• Metabolism of naphthalene, 2-methylnaphthalene, salicylate, and benzoate by Pseudomonas PG: regulation of tangential pathways.
• Comparative biochemical and genetic analysis of naphthalene degradation among Pseudomonas stutzeri strains.
• Plasmid- and chromosome-mediated dissimilation of naphthalene and salicylate in Pseudomonas putida PMD-1.
• Characterization of a novel, cytokine-inducible carboxypeptidase D isoform in haematopoietic tumour cells