Mycobacterium avium subsp. Paratuberculosis and the expression of selected virulence and pathogenesis genes in response to 6°c, 65°c and ph 2.0
AUTOR(ES)
Pribylova, Radka, Kralik, Petr, Donnelly, Neysan, Matiasovic, Jan, Pavlik, Ivo
FONTE
Brazilian Journal of Microbiology
DATA DE PUBLICAÇÃO
2011-06
RESUMO
The aim of this work was to study the expression of selected Mycobacterium avium subsp. paratuberculosis (MAP) genes connected with MAP virulence, adhesion and stress response. The temperature of 6°C and 65°C were chosen with regard to the food industry, storage conditions (refrigerator) and low-temperature pasteurization. A pH of 2.0, using lactic acid, was selected to mimic the natural environment of the stomach. Expression of selected genes was studied using real time reverse transcription PCR on three different MAP isolates. MAP isolates were chosen according to the number of their preceding cultivations. While isolates 8672 and 8819 were previously cultivated only once, MAP isolate 12146 went through four passages. Different expression profiles were observed in each of the three MAP isolates. However, particular similar patterns were observed. SigE, sigF and ahpC were up-regulated, while sigL was down-regulated under temperature stress. Mmp gene was found to be down-regulated under acidic conditions. Low passage isolates (8672 and 8819) showed certain level of acid resistance.
Documentos Relacionados
- Differential Responses of Bovine Macrophages to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium
- Analysis of the Immune Response to Mycobacterium avium subsp. paratuberculosis in Experimentally Infected Calves
- Mycobacterium avium subsp. paratuberculosis in Veterinary Medicine
- Genomic homogeneity between Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis belies their divergent growth rates
- Regulation of Expression of Major Histocompatibility Antigens by Bovine Macrophages Infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium