Mutations in the delta subunit influence the assembly of F1F0 ATP synthase in Escherichia coli.

AUTOR(ES)

Stack, A E

RESUMO

Missense mutations affecting Asp-161 and Ser-163 in the delta subunit of F1F0 ATP synthase have been generated. Although most substitutions allowed substantial enzyme function, the delta Asp-161-->Pro substitution resulted in a loss of enzyme activity. The loss of activity was attributable to a structural failure altering assembly of the enzyme complex.

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