Mutations Affecting the Rossman Fold of Isoleucyl-tRNA Synthetase Are Correlated with Low-Level Mupirocin Resistance in Staphylococcus aureus

AUTOR(ES)

Antonio, Martin

FONTE

American Society for Microbiology

RESUMO

The isoleucyl-tRNA synthetase (ileS) gene was sequenced in toto from 9 and in part from 31 Staphylococcus aureus strains with various degrees of susceptibility to mupirocin. All strains for which the mupirocin MIC was greater than 8 μg/ml contained point mutations affecting the Rossman fold via Val-to-Phe changes at either residue 588 (V588F) or residue 631 (V631F). The importance of the V588F mutation was confirmed by an allele-specific PCR survey of 32 additional strains. Additional mutations of uncertain significance were found in residues clustered on the surface of the IleS protein.

Documentos Relacionados

• Isoleucyl-tRNA Synthetase Mutations in Staphylococcus aureus Clinical Isolates and In Vitro Selection of Low-Level Mupirocin-Resistant Strains  
• High-level mupirocin resistance in Staphylococcus aureus: evidence for two distinct isoleucyl-tRNA synthetases.
• The Staphylococcus aureus ileS gene, encoding isoleucyl-tRNA synthetase, is a member of the T-box family.
• Low-level methicillin resistance in strains of Staphylococcus aureus.
• Mutants of yeast with temperature-sensitive isoleucyl-tRNA synthetases.