Muscle-specific expression of Drosophila hsp70 in response to aging and oxidative stress.
AUTOR(ES)
Wheeler, J C
RESUMO
Induction of Drosophila hsp70 protein was detected during aging in flight muscle and leg muscle in the absence of heat shock, using an hsp70-specific monoclonal antibody, and in transgenic flies containing hsp70-beta-galactosidase fusion protein reporter constructs. While hsp70 and reporter proteins were induced during aging, hsp70 message levels were not, indicating that aging-specific induction is primarily posttranscriptional. In contrast, hsp22 and hsp23 were found to be induced during aging at the RNA level and with a broader tissue distribution. The same muscle-specific hsp70 reporter expression pattern was observed in young flies mutant for catalase (H2O2:H2O2 oxidoreductase, EC 1.11.1.6). In catalase (cat) hypomorphic lines where flies survived to older ages, the time course of hsp70 reporter expression during aging was accelerated, and the initial and ultimate levels of expression were increased. The hsp70 reporter was also induced in young flies mutant for copper/zinc superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1). Taken together, the results suggest that aging-specific hsp70 expression may be a result of oxidative damage.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=40806Documentos Relacionados
- Both muscle-specific and ubiquitous nuclear factors are required for muscle-specific expression of the myosin heavy-chain beta gene in cultured cells.
- Magnetic field stress induces expression of hsp70
- Expressão de hsp70 e hsp83 no desenvolvimento de Drosophila em resposta ao estresse químico causado por disseleneto de difenila e paraquat
- Bradyzoite Development in Toxoplasma gondii and the hsp70 Stress Response
- Expression of hsp22 and hsp70 Transgenes Is Partially Predictive of Drosophila Survival Under Normal and Stress Conditions