MULTIPLICAÇÃO IN VITRO DE MAMOEIRO TAINUNG 01

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Experiments were accomplished in establishment and multiplication medium with the objective of evaluating the micropropagation of the papaya tree (Carica papaya L.) Hybrid Tainung 01 , generation F1, being used apical sections and apical sections of lateral buds of juvenile plants, cultivated in greenhouse. In the MS establishment medium, being used apical sections, different combinations of growth regulators were tested NAA (0.093; 0.931 and 1.862 mg L-1) and kinetin (5.38; 10.76 and 21.52 mg L-1) for identification of the best relationship for the survival, reactivity and callus mass. With the use of 0.093 mg L-1 of NAA combined with 5.38 mg L-1 of kinetin was obtained the best rosette of leaves formation with 100% of cultures reactivate, indicating there to be adaptation of the explants in vitro, besides to smallest callus formation, could be proven with the good reaction of these explants in MS multiplication medium with NAA to 0.093 mg L-1 and BAP to 0.45 mg L-1 during five subculture, with rate of constant multiplication of 5.288:1. In a second stage, with the use of apical sections of lateral buds, the adenine sulfate was evaluated in the multiplication medium. For the preparation of the establishment medium the best combination of the growth regulators was used NAA and kinetin obtained previously, while for the multiplication medium NAA was used to 0.093 mg L-1 and BAP to 0.45 mg L-1, and different levels of adenine sulfate (0; 30; 60; 90 and 120 mg L-1), associated with removal or not of the leaves of the rosette of leaves formed in the establishment medium. The tufts of shoots were subculture every 30 days in smaller tuft and, at the end of the fourth subculture they were made the evaluations of the fresh mass of the aerial part, number of capable shoots (≥ 5 mm) and no capable (<5 mm) to the promoting root formation, and the visual aspect of the tufts of shoots. The best answers for the number of capable shoots to the promoting root formation (≥ 5 mm) they were found with the presence of the leaves, for the treatments 0, 30, 60 and 90 mg L-1 of adenine sulfate. It is recommended to use 30 mg L-1 of adenine sulfate in the multiplication medium for the quality and homogeneity of the produced aerial parts, with prolonged shoots and long petiole, with good pattern for subsequent promoting root formation.

ASSUNTO(S)

carica papaya l. growth regulators adenine sulfate ciencias agrarias mamão - propagação-in vitro tecidos (anatomia e fisiologia) - cultura e meio de cultura multiplication rate reguladores de crescimento establishment medium tissue culture

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