Multiplex Reverse Transcriptase PCR Assay for Simultaneous Detection of Three Fish Viruses†
AUTOR(ES)
Williams, K.
FONTE
American Society for Microbiology
RESUMO
A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=85902Documentos Relacionados
- Reverse transcriptase-PCR assay for detection of hog cholera virus.
- A Nested Reverse Transcriptase PCR Assay for Detection of Small Round-Structured Viruses in Environmentally Contaminated Molluscan Shellfish
- Simultaneous Detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, Three Major Fish Pathogens, by Multiplex PCR
- Evaluation of a Multiplex Real-Time Reverse Transcriptase PCR Assay for Detection and Differentiation of Influenza Viruses A and B during the 2001-2002 Influenza Season in Israel
- A Multiplex Reverse Transcription-PCR Method for Detection of Human Enteric Viruses in Groundwater