Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

AUTOR(ES)

Spositto, F.L.E., Campanerut, P.A.Z., Ghiraldi, L.D., Leite, C.Q.F., Hirata, M.H., Hirata, R.D.C., Siqueira, V.L.D., Cardoso, R. Fressatti

FONTE

Braz. J. Microbiol.

DATA DE PUBLICAÇÃO

2014-09

RESUMO

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.

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