Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
AUTOR(ES)
Evangelista, Cláudia Carolina Silva, Guidelli, Giovanna Vieira, Borges, Gustavo, Araujo, Thais Fenz, Souza, Tiago Alves Jorge de, Neves, Ubiraci Pereira da Costa, Tunnacliffe, Alan, Pereira, Tiago Campos
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
06/11/2017
RESUMO
Abstract The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus.
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