Mu-lac insertion-directed mutagenesis in a pectate lyase gene of Erwinia chrysanthemi.
AUTOR(ES)
Diolez, A
RESUMO
The pelC gene, which encodes one of the five major pectate lyase (PL) isoenzymes in Erwinia chrysanthemi 3937, designated PLc, was subcloned from a hybrid lambda phage into a pBR322 derivative and mutagenized with a mini-Mu-lacZ transposable element able to form fusions to the lacZ gene. One plasmid (pAD1) which had an inactivated pelC gene and a Lac+ phenotype was selected in Escherichia coli. This plasmid was introduced into Erwinia chrysanthemi, and the pelC::mini-Mu insertion was substituted for the chromosomal allele by homologous recombination. This strain lacks the PLc isoenzyme. This Erwinia chrysanthemi strain has a Lac+ phenotype that is inducible by polygalacturonate, as are the wild-type PL activities.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=219219Documentos Relacionados
- Marker-exchange mutagenesis of a pectate lyase isozyme gene in Erwinia chrysanthemi.
- Pectate lyase gene regulatory mutants of Erwinia chrysanthemi.
- The Three-Dimensional Structure of Pectate Lyase E, a Plant Virulence Factor from Erwinia chrysanthemi.
- Regulation of expression of pectate lyase genes pelA, pelD, and pelE in Erwinia chrysanthemi.
- Evidence of homology between the pectate lyase-encoding pelB and pelC genes in Erwinia chrysanthemi.