Morphogenesis of Poliovirus II. Demonstration of a New Intermediate, the Proviron

AUTOR(ES)

Fernandez-Tomas, Carlos B.

RESUMO

Poliovirus-infected cells contain a previously unrecognized particle which appears to be an intermediate in virion synthesis and therefore has been named proviron. It sediments at about 125S, contains the three procapsid proteins, VP-0, VP-1, and VP-3, and has 35S viral RNA. It is disrupted both by sodium dodecyl sulfate and EDTA but the RNA resists digestion by ribonuclease. Pulsechase experiments and studies employing the virus-specific inhibitor, guanidine, all indicate that the proviron is formed by combination of newly made RNA with the procapsid. Cleavage of VP-0 to form VP-2 and VP-4 follows formation of the provirion and would be the final step in poliovirus morphogenesis.

Documentos Relacionados

• Morphogenesis of bulboventricular malformations. II. Observations on malformed hearts.
• Structures of the potassium-saturated, 2:1, and intermediate, 1:1, forms of a quadruplex DNA
• Morphogenesis in Selaginella: II. Auxin Transport in the Root (Rhizophore) 1
• Morphogenesis in Schizophyllum commune. II. Effects of Monochromatic Light 1
• Studies of DNA replication in vivo, II. Evidence for the second intermediate.