Morpho-histological characterization of immature of the bioindicator midge Chironomus sancticaroli Strixino and Strixino (Diptera, Chironomidae)
AUTOR(ES)
Richardi, Vinicius Sobrinho, Vicentini, Maiara, Rebechi, Débora, Fávaro, Luis Fernando, Navarro-Silva, Mário Antônio
FONTE
Rev. Bras. entomol.
DATA DE PUBLICAÇÃO
2015-09
RESUMO
ABSTRACTChironomidae immature are used as bioindicators of sediment quality in aquatic ecosystems and ecotoxicological assays. Histological descriptions for this family are outdated and limited and there are no studies with Neotropical species. The aim of this study was to describe the tissue architecture of several organs of the larva of Chironomus sancticaroli. For the description of the histological pattern, the larvae were fixed in Duboscq solution for insects at 56 °C, followed by routine histologic processing, infiltration in paraffin, and the sections were stained with Hematoxylin–Eosin. After examining the slides, the tube digestive, salivary gland, excretory, nervous, endocrine, circulatory, and integumentary systems and fat body were histologically characterized. The histology allows evaluation of cell morphology, and for being not expensive and easily accessible can be routinely used in biomonitoring. In addition, is a useful tool in ecotoxicological assays and allow to evaluate biomarkers at tissue and cell levels.
Documentos Relacionados
- Growth of immature Chironomus calligraphus (Diptera, Chironomidae) in laboratory conditions
- Factors that alter the biochemical biomarkers of environmental contamination in Chironomus sancticaroli (Diptera, Chironomidae)
- Genotoxic effect of Phenanthrene on Chironomus sancticaroli (Diptera: Chironomidae)
- Setting the reference for the use of Chironomus sancticaroli (Diptera: Chironomidae) as bioindicator: Ontogenetic pattern of larval head structures
- Low malathion concentrations influence metabolism in Chironomus sancticaroli (Diptera, Chironomidae) in acute and chronic toxicity tests