Monoclonal antibodies to the glycoprotein of vesicular stomatitis virus: comparative neutralizing activity.

AUTOR(ES)

Volk, W A

RESUMO

Nineteen independently isolated hybridomas producing monoclonal antibodies to the glycoprotein of vesicular stomatitis virus were isolated and studied for their capacity to neutralize viral infectivity. By measuring competitive binding of 125I-labeled monoclonal antibodies in a radioimmunoassay. 11 different, non-cross-reacting antigenic determinants were identified on the vesicular stomatitis virus G protein. All monoclonal antibodies reacting with determinants 1, 2, 3, and 4 resulted in viral neutralization, whereas those binding to the other seven determinants did not neutralize infectivity. The mixture of two monoclonal antibodies binding to different determinants resulted in a more rapid neutralization than either antibody alone, suggesting that different antibodies can exert a synergistic effect on viral neutralization. Kinetic experiments revealed biphasic neutralization curves similar to those expected for heterologous antibody. No evidence could be obtained to relate biphasic kinetics of viral neutralization to heterogeneous populations either of antibody molecules or of virus. The possible significance of the kinetic data with monoclonal antibodies is discussed.

Documentos Relacionados

• Amino-terminal mutation of the vesicular stomatitis virus glycoprotein does not affect its fusion activity.
• Neutralizing monoclonal antibodies to hepatitis A virus: partial localization of a neutralizing antigenic site.
• Assembly of vesicular stomatitis virus: distribution of the glycoprotein on the surface of infected cells.
• The Glycoprotein of Vesicular Stomatitis Virus Is the Antigen That Gives Rise to and Reacts with Neutralizing Antibody
• Monoclonal antibodies to newcastle disease virus: delineation of four epitopes on the HN glycoprotein.