Molecular Weight Determination of Sendai RNA by Dimethyl Sulfoxide Gradient Sedimentation
AUTOR(ES)
Kolakofsky, Daniel
RESUMO
The molecular weight of the large RNA of Sendai virus has been determined by sedimentation analysis in sucrose gradients containing 99% dimethyl sulfoxide (DMSO) to be 2.3 × 106. Sendai RNA recovered from 99% DMSO was found to cosediment with nondenatured Sendai RNA at 46 to 48s in ordinary sucrose gradients. The molecular weight value of 2.3 × 106 is considerably smaller than the estimates of 6 × 106 to 7 × 106 determined under nondenaturing conditions, suggesting a unique structure for Sendai RNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355155Documentos Relacionados
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