Molecular studies of Anopheles albitarsis and Anopheles triannulatus (Diptera: Culicidae) captured in flood plain breeding sites in Mato Grosso do Sul, Brazil / Estudos moleculares de Anopheles albitarsis e Anopheles triannulatus (Diptera: Culicidae) capturados em criadouro na planície de inundação do Mato Grosso do Sul, Brasil

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

Malaria is a severe disease whose vectors are mosquitoes belonging to the genus Anopheles. This genus contains 500 species of which approximately 45 are responsible for the worldwide transmission of these parasites. Some of these vectors belong to cryptic species such as those of the Albitarsis and Triannulatus Complex. The Albitarsis Complex is composed of four species: An. albitarsis s.s., An. albitarsis B, An. marajoara and An. deaneorum. The Triannulatus Complex contains three species: An. triannulatus, An. halophylus and An. triannulatus C. We used molecular techniques to differentiate these cryptic species. Thus, DNA of An. albitarsis s.l and An. triannulatus s.l, captured at the Porto Primavera Dam had their ITS2 and their mtDNA, ND4 genes amplified. All samples were analyzed by the RFLP technique. Some samples were directly sequenced while others were cloned for subsequent sequencing for species confirmation. Within the Albitarsis Complex, 62.85% were identified as An. deaneorum by RFLP-ITS2. The remaining were amplified by RFLP-ND4 and identified as An. albitarsis s.s. However the results of sequencing of the samples of the Albitarsis Complex did not overlap entirely with those deposited in the GeneBank since those amplified by RFLP-ITS2 were identified as An. albitarsis s.s. and An. deaneorum while those obtained by RFLP-ND4 were identified as An. albitarsis B. Also RFLP-ITS2 of A. triannulatus s.l. contained polymorphic regions among different species. By sequencing, these samples were similar to those deposited in the GeneBank. The use of molecular markers did, in some instances help to distinguish species within cryptic complexes, however other markers need to be evaluated to elucidate further identification of the Albitarsis Complex.

ASSUNTO(S)

dna espaçador ribossômico anopheles albitarsis polymerase chain reaction dna ribosomal spacer dna mitocondrial anopheles triannulatus anopheles albitarsis malária reação em cadeia da polymerase anopheles triannulatus insect vectors dna mitochondrial insetos vetores malaria

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