Molecular signature of immunological mechanism behind impaired endometrial receptivity in polycystic ovarian syndrome
AUTOR(ES)
Amjadi, Fatemehsadat; Zandieh, Zahra; Mehdizadeh, Mehdi; Ajdary, Marziyeh; Aghamajidi, Azin; Raoufi, Ehsan; Aflatoonian, Reza
FONTE
Archives of Endocrinology and Metabolism
DATA DE PUBLICAÇÃO
2022
RESUMO
ABSTRACT Objective: Despite the treatment of anovulation, infertility is still one of the main complications in PCOS women during reproductive age, which appears to be mainly due to impaired uterine receptivity. This study investigated the transcriptome profiles of endometrium in PCOS patients and healthy fertile individuals as the control group. Material and methods: Total mRNA was extracted from endometrial tissues of PCOS patients (n = 12) and healthy fertile individuals (n = 10) during the luteal phase. After cDNA synthesis, PCR array was performed using Human Female Infertility RT² Profiler PCR Array kit (Qiagen, Cat. No: PAHS-164Z) for evaluating expression of 84 genes contributing to the female infertility. Results: PCR Array data analysis identified significantly greater expression of CSF, IL11, IL15, IL1r1, IL1b, TNF, LIF, TNFRSF10B, TGFβ, C3, ITGA4 (Cd49d), SPP1, and Calca in PCOS women than in controls (P < 0.05). However, the expression of LIFR, C2, CD55, CFD, CALCA, LAM1, LAMC2, MMP2, MMP7, MMP9, ESR, SELL, ITGB3, and VCAM1 was significantly lower in PCOS group than in controls (P < 0.05). The results revealed dysregulation of immune-inflammatory molecules, complement activation and downregulation of IGF-I as well as adhesion molecules in PCOS group. Conclusion: The findings of this study indicated some potential causes of reduced receptivity of endometrium thus compromising the fertility in PCOS patients.
Documentos Relacionados
- Metabolic syndrome in infertile women with polycystic ovarian syndrome
- The Prevalence of Metabolic Syndrome in the Different Phenotypes of Polycystic Ovarian Syndrome
- Endothelial Dysfunction and Pulse Wave Reflection in Women with Polycystic Ovarian Syndrome
- Endothelial Dysfunction and Pulse Wave Reflection in Women with Polycystic Ovarian Syndrome
- Excessive Ovarian Production of Nerve Growth Factor Facilitates Development of Cystic Ovarian Morphology in Mice and Is a Feature of Polycystic Ovarian Syndrome in Humans