Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

AUTOR(ES)

Ferreira, Tauana de Sousa, Minuzzi-Souza, Thaís Tâmara Castro, Andrade, Andrey José de, Coelho, Thais Oliveira, Rocha, Douglas de Almeida, Obara, Marcos Takashi, Hecht, Mariana, Nitz, Nadjar, Gurgel-Gonçalves, Rodrigo

FONTE

Rev. Soc. Bras. Med. Trop.

DATA DE PUBLICAÇÃO

2015-12

RESUMO

Abstract: INTRODUCTION : This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. METHODS : Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. RESULTS : PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. CONCLUSIONS : Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.

Documentos Relacionados

• Phlebotomine sand flies in the State of Piauí, Brazil (Diptera: Psychodidae: Phlebotominae)
• Phlebotomine sand flies (Diptera: Psychodidae) of the state of Minas Gerais, Brazil
• Frequency and diversity of phlebotomine sand flies (Diptera: Psychodidae) in Sinop, State of Mato Grosso, Brazil
• Phlebotomine sand flies (Diptera: Psychodidae) of the province of Chaco, Argentina
• Bionomics of phlebotomine sand flies (Diptera: Psychodidae) in the province of Al-Baha, Saudi Arabia