Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA)
AUTOR(ES)
Bustamante, Ana V., Lucchesi, Paula M.A., Parma, Alberto E.
FONTE
Brazilian Journal of Microbiology
DATA DE PUBLICAÇÃO
2009-12
RESUMO
The aim of this work was to adapt described MLVA protocols to the molecular typing and characterization of VTEC O157:H7 isolates from Argentina. Nine VNTR loci were amplified by PCR showing diversity values from 0.49 to 0.73. Nine MLVA profiles were observed and the cluster analysis indicated both unrelated and closely related VTEC O157:H7 strains. In spite of the limited number of isolates studied, the panel of VNTR used made it possible to perform a first approach of the high genetic diversity of native strains of O157:H7 by MLVA.
Documentos Relacionados
- DNA fingerprinting of Shiga-toxin producing Escherichia coli O157 based on Multiple-Locus Variable-Number Tandem-Repeats Analysis (MLVA)
- Escherichia coli O157:H7
- Characterization of Unexpected Growth of Escherichia coli O157:H7 by Modeling
- Phenotypic and Molecular Analysis of Tellurite Resistance among Enterohemorrhagic Escherichia coli O157:H7 and Sorbitol-Fermenting O157:NM Clinical Isolates
- Molecular typing of enterohemorrhagic Escherichia coli O157:H7 isolates in Japan by using pulsed-field gel electrophoresis.