Molecular characterization of a phytoplasma associated with a commercial variety of Momordica charantia
AUTOR(ES)
Munhoz, Elizeu Merizio
FONTE
Sci. agric. (Piracicaba, Braz.)
DATA DE PUBLICAÇÃO
2019-05
RESUMO
ABSTRACT: Momordica charantia (bitter melon) presents two distinct types or varieties, known as wild type and commercial type. Plants of the wild type are hosts of a phytoplasma of the group 16SrIII-J, which is associated with a disease known as witches’ broom. However, this disease has not yet been reported in commercial bitter melon. Thus, symptomatic plants of the commercial type were analyzed in order to demonstrate the association between phytoplasmas and disease. In further assays, strains found in symptomatic plants of the commercial type were subjected to analysis of sequences of the secY gene to determine the extent of genetic diversity. Amplification of DNA fragments from genes 16Sr rRNA (1.2Kb) and secY (1.6Kb) revealed association of phytoplasma with symptomatic plants of the commercial type. Virtual Restriction Fragment Length Polymorphism (RFLP) analysis identified this phytoplasma as a member of the subgroup 16SrIII-J. Phylogenetic analysis showed that the phytoplasma was closely related to the representative of the 16SrIII-J subgroup. Molecular analysis indicated that the secY gene, in spite of the greater genetic variation compared with 16S rRNA gene, did not separate strains of the phytoplasma of the subgroup 16SrIII-J among those strains present in M. charantia.
Documentos Relacionados
- Efeitos de Momordica charantia L. em ratos diabéticos
- Ensaios biolÃgicos para avaliar o efeito radioprotetor da Momordica Charantia L
- Detection and molecular characterization of cactus witches'-broom disease associated with a group 16SrII phytoplasma in northern areas of China
- Atividade antifúngica de extratos de Momordica charantia L. sobre Colletotrichum musae
- Avaliação biométrica de caracteres do melão de São Caetano (Momordica charantia L)