Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm.

AUTOR(ES)

CARNEIRO, A.A.

FONTE

Pesquisa Agropecuária Brasileira

DATA DE PUBLICAÇÃO

2011

RESUMO

The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.

ASSUNTO(S)

spodoptera frugiperda entomopathogenic fungi rapd rdna-its fungo entomopatogênico

Documentos Relacionados

• Molecular Characterization and Identification of Biocontrol Isolates of Trichoderma spp.
• Molecular characterization of Candida spp. isolates from patients with bloodstream infections
• Genotyping isolates of Beauveria spp. by rDNA-ITS sequencing and RAPD markers.
• Molecular and Phenotypic Characterization of Pseudomonas spp. Isolated from Milk
• Naturally occurring deletions in the centisome 63 pathogenicity island of environmental isolates of Salmonella spp.