Modulation of T-lymphocyte differentiation antigens: potential relevance for multiple sclerosis.
AUTOR(ES)
Antel, J
RESUMO
Effects of the anti-T-cell monoclonal antibodies OKT3, OKT5, and OKT8 on T-cell surface properties and cell functions were evaluated. Incubation of mononuclear cells isolated from peripheral blood for 48 hr with each monoclonal antibody in the absence of complement resulted in modulation of their respective surface antigens; i.e., the number of cells detected by immunofluorescence as positive for the T3, T5, and T8 surface antigens was reduced. T3, T5, and T8 antigens modulated independently. A radiolabeled second antibody technique confirmed modulation by OKT3 and OKT8 and indicated that T-cell differentiation antigens can regenerate in culture. Incubation of mononuclear cells with OKT3 increased the number of sheep erythrocyte-binding lymphocytes (E+-rosetting cells) and markedly increased the number of avidly E+-rosetting cells. Incubation with OKT8 reduced the number of E+- and of avidly E+-rosetting cells. OKT3 induced both mitogenic reactivity and suppressor cell activity; cells modulated by OKT8 exhibited reduced mitogenic reactivity and reduced suppressor cell function. The decreases in total T cells, in avid T cells, in suppressor cell number, and in suppressor cell function that follow modulation by OKT8 mimic changes observed in multiple sclerosis patients.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=346409Documentos Relacionados
- Quantitation of potential T-lymphocyte function in rats.
- Heterogeneity of thymic epithelial cells in promoting T-lymphocyte differentiation in vivo.
- T-lymphocyte subpopulations in uveitis.
- T-lymphocyte differentiation in vitro in severe combined immunodeficiency. Defects of stem cells.
- Common Antiviral Cytotoxic T-Lymphocyte Epitope for Diverse Arenaviruses†