Modulação da AMP-activated protein kinase (AMPK) em hipotalamo de ratos wistar submetidos ao exercicio / Exercises alters AMPK activity in the hypothalamus of wistar rats

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

AMP-activated protein kinase (AMPK) and mammalian Target of Rapamycin (mTOR) are key regulators of cellular energy balance and of the effects of leptin on food intake. Acute exerci se is associated with increased sensitivity to the effects of leptin on food intake in an IL-6-dependent manner. To determine whether exerci se ameliorates the AMPK and mTOR response to leptin in the hypothalamus in an IL-6-dependent manner, rats performed two 3-h exercise bouts, separated by one 45-min rest períod. Intracerebroventrícular IL-6 infusion reduced food intake and pretreatment with AMPK activators and mTOR inhibitors prevented IL-6-induced anorexia. Activators of AMPK increased food intake in control rats to a greater extent than that observed in exercised ones, whereas inhibitors of AMPK had the opposite effect. Exercise was associated with both reduced phosphorylation of the AMPK/ ACC signaling pathway and increased phosphorylation of proteins involved in mTOR signal transduction Ín the hypothalamus. The regulatory role of IL-6 in mediating the modulation in AMPK and mTOR pathways in the hypothalamus was also investigated. Treatment with leptin reduced food intake in exercised rats that were pretreated with vehicle, although no increase in sensitivity to leptin-induced anorexia after pretreatment with anti-IL6 antibody, AICAR or Rapamycin was detected. Improved responses of AMPK and mTOR to leptin may contribute to the appetite suppressive actions of exercise

ASSUNTO(S)

appetite regulation interleucina-6 regulação do apetite neuronios neurons adenosina trifosfato natação interleukin-6 swimming atp (bioquimica)

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