Modification of the erythroid transcription factor GATA-1 by SUMO-1
AUTOR(ES)
Collavin, Licio
FONTE
National Academy of Sciences
RESUMO
The activity of transcription factors is tightly modulated by posttranslational modifications affecting stability, localization, and protein–protein interactions. Conjugation to SUMO is a reversible posttranslational modification that has been shown to regulate important transcription factors involved in cell proliferation, differentiation, and tumor suppression. Here, we demonstrate that the erythroid transcription factor GATA-1 is sumoylated in vitro and in vivo and map the single lysine residue involved in SUMO-1 attachment. We show that the nuclear RING finger protein PIASy promotes sumoylation of GATA-1 and dramatically represses its transcriptional activity. We present evidence that a nonsumoylatable GATA-1 mutant is indistinguishable from the WT protein in its ability to transactivate a reporter gene in mammalian cells and in its ability to trigger endogenous globin expression in Xenopus explants. These observations open interesting questions about the biological role of this posttranslational modification of GATA-1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=428439Documentos Relacionados
- Ligand-dependent repression of the erythroid transcription factor GATA-1 by the estrogen receptor.
- Transactivation of erythroid transcription factor GATA-1 by a myb-ets-containing retrovirus.
- Self-association of the erythroid transcription factor GATA-1 mediated by its zinc finger domains.
- Transcription factor GATA-1 permits survival and maturation of erythroid precursors by preventing apoptosis.
- Positive regulators of the lineage-specific transcription factor GATA-1 in differentiating erythroid cells.
