Model of human skin (dermis + epidermis) reconstructed in vitro / Modelo de pele humana (derme + epiderme) reconstruida in vitro

AUTOR(ES)

Luis Ricardo Martinhão Souto

DATA DE PUBLICAÇÃO

2005

RESUMO

The technique to obtain human skin presenting dermis and epidermis reconstructed from cells isolated from patients allows the performance of autologous grafts of skin reconstructed in laboratory (in vitro) on patients with scarce donor sites, in addition to permitting trials with chemical substances and drugs no more in vivo, but in vitro. It is possible to obtain a sufficient number of cells from human fibroblast culture that can be injected in bovine collagen type I matrix and kept submerged in a specific culture medium for fibroblasts. This will permit the formation of human dermis reconstructed in vitro. On this dermis, through culture of human keratinocytes and melanocytes, a differentiated epidermis is formed, leading to the creation of human skin reconstructed in vitro, composed of associated dermis and epidermis. This human skin is histologically formed in the same way as human skin in vivo. Collagen tissue can be identified in the dermis, with its cells and extracellular matrix organized in parallel to the epidermis, which is developed in several layers

ASSUNTO(S)

epidermis grafting of skin fibroblastos pele - enxerto epiderme colageno collagen fibroblastos

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