Minimal Requirements for Commitment to Sporulation in Bacillus megaterium

AUTOR(ES)

Greene, Richard A.

RESUMO

Commitment to sporulation was examined by means of both endotrophic sporulation and rejuvenation experiments. In both cases, a point of commitment to the completion of sporulation occurred at stage II. With 2 × 108 cells/ml, the process by which commitment occurred required 0.5 mm Mg2+, 10.0 mm phosphate, and an energy source (minimum of 5.0 mm acetate). For completion of sporulation and formation of normal, heat-resistant refractile spores, 0.3 mm Ca2+ was required. Except for this Ca2+ requirement, the completion of sporulation after commitment was independent of the nature of the surrounding medium. Some aspects of the utilization of these required substances were examined.

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