Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA.
AUTOR(ES)
Hayakawa, Y
RESUMO
A mini-F-coded protein, named F2 protein, binds specifically to mini-F DNA. This protein has a molecular weight of 37,000 and is coded by the A2 segment of the mini-F genome (47.3 to 49.4 kilobases on the F coordinate map). The binding site is located also in the A2 segment of mini-F. This binding site is lost by spontaneous deletion when the A2 segment alone, but not A2 together with its neighboring segment, is cloned in a multicopy plasmid pBR322. These data are discussed in connection with incompatibility and plasmid stability.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=219120Documentos Relacionados
- Bidirection replication from a unique origin in a mini-F plasmid.
- Mini-F plasmid genes that couple host cell division to plasmid proliferation.
- DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal region.
- Analysis of mini-F plasmid replication by transposition mutagenesis.
- A single 43-bp sopC repeat of plasmid mini-F is sufficient to allow assembly of a functional nucleoprotein partition complex.