Microsticks as solid-phase carriers for enzyme-linked immunosorbent assays.

AUTOR(ES)

Shekarchi, I C

RESUMO

Microsticks are machine-tooled or molded pegs of plastic or stainless steel which were developed as solid-phase carriers for the enzyme-linked immunosorbent assay (ELISA). They consist of a stem, which can be coated with plastic to be used as the reactive surface, and a shaft designed for easy handling and labeling and for positioning the sticks in microtiter wells and transfer plates. Microsticks permit a wide selection of coating materials and afford the user greater control over quality and standardization of the solid-phase surface. Polycarbonate and nitrocellulose coatings were tested in ELISAs for antibodies to measles virus, toxoplasma, and human gamma globulin. The Microsticks were found to give reproducible, sensitive, and specific ELISA results and minimized problems due to lot-to-lot and batch-to-batch variations found with other plastic carriers.

Documentos Relacionados

• Polycarbonate-coated microsticks as solid-phase carriers in an enzyme-linked immunosorbent assay for rubella antibody.
• A simple washing technique for solid-phase radioimmunoassays and enzyme-linked immunosorbent assays.
• Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A virus.
• Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A-specific immunoglobulin M.
• Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus.