Microcalorimetric Study of Glucose Permeation in Microbial Cells1
AUTOR(ES)
Belaich, Jean-Pierre
RESUMO
A microcalorimetric method for measuring the influence of extracellular glucose concentration on the rate of catabolism is described. This method has been applied to anaerobically growing cultures of Zymomonas mobilis and of a respiratory-deficient (“petite”) mutant of Saccharomyces cerevisiae (strain YFa). The Michaelian kinetics recorded with both organisms were apparently related to glucose transport. With Z. mobilis, it was found that, in the range of glucose concentrations at which this organism was growing exponentially, cell activity was limited by the maximal rate of the catabolic enzymes; at lower concentrations, glucose transport was the rate controlling step. The metabolic activity of yeast always depended on external glucose concentration; when this was lowered under a threshold, a change of kinetics took place. The microcalorimetric method described seems to be widely applicable to kinetic studies of the permeation of metabolizable substrates in microorganisms.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=252207Documentos Relacionados
- The Elicitor Cryptogein Blocks Glucose Transport in Tobacco Cells1
- Ethylene-Mediated Phospholipid Catabolic Pathway in Glucose-Starved Carrot Suspension Cells1
- TURBIDITY CHANGE DURING GLUCOSE PERMEATION IN ESCHERICHIA COLI1
- COMPARATIVE STUDY OF THE BINDING OF SYNNEMATIN AND PENICILLIN BY BACTERIAL CELLS1
- VIRUSES, GENES, AND CELLS1