Microbe forensics: Oxygen and hydrogen stable isotope ratios in Bacillus subtilis cells and spores
AUTOR(ES)
Kreuzer-Martin, Helen W.
FONTE
The National Academy of Sciences
RESUMO
Bacillus subtilis, a Gram-positive, endospore-forming soil bacterium, was grown in media made with water of varying oxygen (δ18O) and hydrogen (δD) stable isotope ratios. Logarithmically growing cells and spores were each harvested from the cultures and their δ18O and δD values determined. Oxygen and hydrogen stable isotope ratios of organic matter were linearly related with those of the media water. We used the relationships determined in these experiments to calculate the effective whole-cell fractionation factors between water and organic matter for B. subtilis. We then predicted the δ18O and δD values of spores produced in nutritionally identical media and local water sources for five different locations around the United States. Each of the measured δ18O and δD values of the spores matched the predicted values within a 95% confidence interval, indicating that stable isotope ratio analyses may be a powerful tool for tracing the geographic point-of-origin for microbial products.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=298684Documentos Relacionados
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