Micobacteriose parotidea na AIDS em fase avançada

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

The aim of this work was to study the parotid mycobacteriosis by histopathologic analysis and to evaluate diagnosis methods: Ziehl-Neelsen staining, immunohistochemistry reaction, Polymerase Chain Reaction (PCR) and Ligase Chain Reaction (LCR). Ten cases of parotid mycobacteriosis in advanced autopsied AIDS patients were selected. Methods for detection of genus Mycobacterium were conducted (Ziehl-Neelsen staining, BCG immunohistochemistry reaction and PCR for amplification of small region of gene responsible for conserved protein in the genus Mycobacterium. Methods for qualitative detection of “M. tuberculosis complex” (LCR and PCR) and Mycobacterium avium (PCR) were also conducted. The intraparotid lymph nodes were mainly involved (10 cases) and the glandular parenchyma was affected in only 3 cases. Two patterns of parotid mycobacteriosis were diagnosed: 8 cases showed a chronic non-caseating partly granulomatous inflammation and 2 cases displayed an infiltrate of foamy macrophages, full of mycobacteria, scant areas of necrosis and no granuloma formation. Evaluating the immunological profile of parotid mycobacteriosis, macrophages were more prevalent, followed by TCD8 lymphocytes, B lymphocytes and TCD4 lymphocytes. The three techniques performed for genus detection showed similar sensitivity and were positive for all cases (10 cases). “M. tuberculosis complex” was detected in 5 cases by LCR and in 8 cases by PCR. The M. avium infection was detected in 1 case only, which was also positive for “M. tuberculosis complex” showing a co-infection result. The PCR technique for “M.tuberculosis complex” detection showed better results than LCR. PCR was an efficient diagnosis method for identification of Mycobacterium species in paraffin-embedded parotid mycobacteriosis.

ASSUNTO(S)

diagnostico aids (doença) micobacterias

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