Methyl-directed repair of frameshift mutations in heteroduplex DNA.
AUTOR(ES)
Dohet, C
RESUMO
DNA heteroduplexes with single unpaired bases of the four different kinds were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system can recognize and repair heteroduplexes with single unpaired bases--i.e., frameshift/wild-type heteroduplexes. The repair of a particular strand of the heteroduplex is inhibited by full methylation of the adenines in the GATC sequences of that strand. Thus, it appears that the E. coli mismatch repair system can act on newly synthesized DNA strands to remove replication errors involving the insertion or deletion of a single base.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=323520Documentos Relacionados
- Methyl-directed repair of frameshift heteroduplexes in cell extracts from Escherichia coli.
- Methyl-directed repair of DNA base-pair mismatches in vitro.
- Effects of High Levels of DNA Adenine Methylation on Methyl-Directed Mismatch Repair in ESCHERICHIA COLI
- Recombinagenic Processing of Uv-Light Photoproducts in Nonreplicating Phage DNA by the Escherichia Coli Methyl-Directed Mismatch Repair System
- The Escherichia coli Methyl-Directed Mismatch Repair System Repairs Base Pairs Containing Oxidative Lesions