Metabolismo do dibenzotiofeno por Cunninghamella elegans associados aos estudos morfolÃgicos e ultraestruturais

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Considering the biotechnological potential of Cunninghamella elegans related to recalcitrant compounds in the environment the aim of this work was to evaluate the dibenzothiophene (DBT) degradation in association with morphological and ultrastructral aspects. Samples treated with DBT were submitted to gas chromatography coupled to mass spectrometry - GC-MS. The metabolites related to DBT degradation obtained were 5-oxide dibenzothiophene, 5,5-dioxide dibenzothiophene, 2- hydroxybifenyl, results which suggest the utilization of 4S metabolic pathway. A second metabolic pathway is also suggested by angular dioxigenation, in which DBT is used as carbon source, and remove the sulfur in sulphite our sulphate form, and the final intermediate is the benzoic acid. The study also revealed that C. elegans growth profile was stimulated by all DBT concentrations. The total extracellular proteins varied according to DBT concentrations related to control. The cellular morphology study revealed variations in the branching pattern, form, with hyphae more short, thin, bifurcated, bilateral or unilateral and also demonstrated the presence of protuberances related to DBT concentration. The fluorescence microscopy revealed variations in actin cytoskeleton related to distribution, form and localization of reaction products. The use of scanning electron microscopy revealed alterations induced by DBT concentrations related to electrondensity, branching and presence of cellular bodies. The transmission electron microscopy revealed variations related to the presence of electrondense bodies, vacuoles, mitochondria and alterations related to cell wall electrondensity and cellular texture. The catalase citochemical analysis revealed variations in the intensity and distribution of reaction products in the cell wall and cytoplasm of samples treated with DBT. This study revealed the biotechnological potential of C. elegans in the DBT degradation

ASSUNTO(S)

ultraestrutura catalase cunninghamella elegans morfologia biodessulfurizaÃÃo ultrastructure microbiologia dibenzothiophene biodesulfurization catalase cunninghamella elegans dibenzotiofeno morphology

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