Mechanism of control of cytochrome oxidase activity by the electrochemical-potential gradient.
AUTOR(ES)
Brunori, M
RESUMO
Cytochrome c oxidation by bovine cytochrome oxidase embedded into liposomal vesicles with high respiratory control ratio (RCR = 6-10) has been studied by rapid-mixing experiments in the presence and absence of different ionophores. Kinetic analysis of the reaction indicates a linkage between the intrinsic activity of the enzyme, the efficiency of coupling and the electrochemical potential across the membrane. A simple model, based on two allosteric states with different catalytic properties in rapid equilibrium, is presented and successfully applied in the simulation of the observed time-course.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554511Documentos Relacionados
- Escherichia coli adenylate cyclase complex: regulation by the proton electrochemical gradient.
- Dynamical change of mitochondrial DNA induced in the living cell by perturbing the electrochemical gradient.
- Amino acid transport in the thermophilic anaerobe Clostridium fervidus is driven by an electrochemical sodium gradient.
- Direct Demonstration of a Growth-Induced Water Potential Gradient.
- Dissipation of the Proton Electrochemical Potential in Intact Chloroplasts (II. The pH Gradient Monitored by Cytochrome f Reduction Kinetics).