Measurement of Size Distributions of Bacterial Cells
AUTOR(ES)
Harvey, R. J.
RESUMO
Harvey, R. J. (University of California, Davis), and Allen G. Marr. Measurement of size distributions of bacterial cells. J. Bacteriol. 92:805–811. 1966.—Apparatus for the automatic determination of the volume distribution of particles by measurement of the amplitude of pulses generated in a Coulter transducer is described. Distributions of volume estimated by direct measurement of pulse amplitude are distorted by coincidence. Differentiation and integration of the pulses followed by automatic pulse-height analysis permit precise measurement of volume of latex spheres and of bacteria over a range of at least 0.25 to 20 μ3. The apparatus is also capable of accurate determination of particle concentration over a wide range. Other advantages are the speed of both measurement and data processing.
