Mastócitos no lobo ventral da próstata, no epidídimo e no testículo de ratos UChB (consumidores voluntários de etanol a 10%) / Mast cells in the ventral prostate, epididymis and testis of UchB - ethanol preferring rats (10% v/v high ethanol voluntary intake)
AUTOR(ES)
Leonardo de Oliveira Mendes
FONTE
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia
DATA DE PUBLICAÇÃO
29/03/2010
RESUMO
Alcoholism has reached alarming proportions throughout the world, but there is little research on the inflammatory effect of ethanol in the reproductive organs. The mast cell, which is one of the main cells involved in inflammatory processes, is known to have two subtypes in rodents, connective (CTMC) and mucosal (MMC) tissue. Hence, the aim of this work is to locate and quantify mast cells in the ventral lobe of the prostate, in the testis and epididymis of UChB rats and then assess if there is a relation between ethanol ingestion and the number of mast cells in these organs. The animals were divided into three experimental groups (n = 10/group): UChB rats that received 10% ethanol administration (UChBet); UChB rats without ethanol administration (UChBco); and Wistar rats (W). Samples of the ventral lobe of the prostate, right testis and right epididymis were collected after 180 days of experiments and processed for light microscopy analysis. The material was stained with toluidine blue for identification of total mast cells and immunohistochemistry was performed for CTMC identification. The animals who received ethanol exhibited high density of degranulated mast cells in the ventral prostate. There wasnt statistic difference in the number of mast cells, intact e degranulated, in the testis. The epididymis showed differences according to the analyzed segment. There was a higher density of intact and degranulated mast cells in UChBet when compared to UChBco in the initial segment. A high density of intact mast cells in the epididymal caput was observed in W, with no difference in the degranulated cells regarding this region. There was no difference between groups for intact mast cells in the region of the epididymal corpus, but the number of degranulated mast cells was higher in animals that received ethanol. There was no difference regarding the number of intact mast cells in the epididymal cauda, but the number of degranulated mast cells was higher in animals that received ethanol. There was a greater number of CTMC in the prostate of UChBet rats and a greater amount of MMC as well. There was higher amount of CTMC in the W rat testis and, proportionally, greater amount MMC in UChB groups. Staining for CTMC in epididymis of UChB animals was lower than W animals, which allows the establishment of a higher proportion of MMC in these animals. We concluded that ethanol increases the number of degranulated mast cells and MMC, and thus, it could be promoting inflammation in the prostate, epididymis and testis of UChB rats.
ASSUNTO(S)
rato próstata - lobo ventral testículos epidídimo Álcool mastócitos rats prostate testis epididymis mast cells
ACESSO AO ARTIGO
http://libdigi.unicamp.br/document/?code=000770089Documentos Relacionados
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