Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population.

AUTOR(ES)
FONTE

Crop Breeding and Applied Biotechnology

DATA DE PUBLICAÇÃO

2011

RESUMO

A doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPS162072 (?AGGGGGTTCC?) at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC3 F1 population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis.

ASSUNTO(S)

duplo haplóide arroz oryza sativa antera brusone doença de planta marcador molecular rice doubled haploids anther culture magnaporthe grisea

ACESSO AO ARTIGO

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