Marcadores tumorais bioquímicos e imunocitoquímicos em efusões neoplásicas caninas / Biochemical and immunocytochemical tumor markers in canine neoplastic effusions

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

2012

RESUMO

The cavity effusions frequently occur in the clinical routine of dogs. In most cases the effusions are benign caused by circulatory system disorders. Neoplasms are common causes of effusions in dogs, however not always the tumor cells are found in cytopathologycal analysis. The dosage of tumor markers is an alternative to make the neoplastic effusion diagnosis more accurate. The aims of this work were to determine the following biochemical tumor markers: carcinoembryonic antigen (CEA), cancer antigen 72-4 (CA 72-4) and cytokeratin fragment 21-1 (CYFRA 21-1), which have not had their performance evaluated in canine neoplastic and non-neoplastic effusions, as well as immunocytochemical markers including two new primary antibodies (MOC-31 and D2-40) for differentiation between carcinoma and mesothelioma tumors. Thrirtytwo samples of abdominal and thoracic cavity fluids, from the clinical care of the Veterinary Hospital of the Federal University of Rio Grande do Sul were analyzed. According to the cytopathology test and patient¿s clinical record the effusions were classified in two groups: neoplastic or non-neoplastic. The tumor markers measurement was performed by sandwich enzyme immunoassay (ELISA) according to the manufacturer instructions. Fourteen neoplastic samples were used for the immunocytochemical tests. The tests were performed by streptavidin-biotin method linked to peroxidase or to alkaline phosphatase using the DAB chromogen. The tumor markers CEA and CA 72-4 had no significant results for differentiating between neoplastic and non neoplastic effusions, whereas the tumor marker CYFRA 21-1 obtained 70% of sensibility, 94% of specificity and 81% of accuracy for the neoplastic diagnosis. In all neoplastic samples the immunocytochemical and cytological tests were compatible, which make valid their use for standardization of those new antibodies for the canine species. This study demonstrated that new tumor markers both biochemical and immunocytochemical could be used in the canine neoplastic diagnosis. The tumor marker CYFRA 21-1 must be used for the canine species, and the antibodies MOC-31 and D2-40 must be included in routine immunocytochemistry panel for the differenciation between carcinoma and mesothelioma in neoplastic effusions.

ASSUNTO(S)

neoplastic effusions cães : doenças : diagnóstico canine imunocitoquimica neoplasias : diagnóstico tumor markers immunocytochemistry bioquimica veterinaria

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