MarcaÃÃo do alelo de resistÃncia do feijÃo comum à mancha angular por meio de microssatÃlite e RAPD / Tagging resistance allele of the common bean to angular leaf spot by microsatellite and RAPD markers.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

Angular leaf spot (Phaeisariopsis griseola) is one of the most important diseases of common bean, since it is widespread all over the country in Brazil and causes great losses in grain yield. The objective of this estudy was to identify the resistant allele from the andean Jalo cultivar, with RAPD and SSR markers, in order to assist selection of resistant genotypes. The resistant line ESAL 550 and the susceptible cultivar Carioca MG were crossed to generate the F1 and F2 populations. 120 F2;3 plants were evaluated. The DNA of the 10 most resistant plants were bulked, and the same was done with the DNA of the 10 most susceptible, generating two contrasting bulks. Using these two bulks and the F2 population, two RAPD and one SSR markers were found to be linked to the reaction gene. The SSR marker was amplified by the primer PV-atct001 282, and the recombination frequency was 0.0702 (SE=0.0005; 95%; CI= 0.029-0.11). This is the most useful marker for the indirect selection of resistant plants in segregating populations because the error in the selection will be small. The RAPD markers were amplified by the primer OPP07 linked in coupling phase and OPBB04 linked in repulsion phase to the resistant allele, respectively, with recombination frequencies of 0.192 and 0.327. Therefore, these RAPD markers are not so useful in assisting selection, because they are too far from the resistant allele.

ASSUNTO(S)

microssatÃlite genetica vegetal rapd plant breeding melhoramento genÃtico vegetal alelo de resistÃncia resistance allel common bean feijÃo rapd microssatellite

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