Mapping segmental and sequence variations among laboratory mice using BAC array CGH

AUTOR(ES)

Snijders, Antoine M.

FONTE

Cold Spring Harbor Laboratory Press

RESUMO

We used arrays of 2069 BACs (1303 nonredundant autosomal clones) to map sequence variation among Mus spretus (SPRET/Ei and SPRET/Glasgow) and Mus musculus (C3H/HeJ, BALB/cJ, 129/J, DBA/2J, NIH, FVB/N, and C57BL/6) strains. We identified 80 clones representing 74 autosomal loci of copy number variation (|log2ratio| ≥ 0.4). These variant loci distinguish laboratory strains. By FISH mapping, we determined that 63 BACs mapped to a single site on C57BL/6J chromosomes, while 17 clones mapped to multiple chromosomes (n = 16) or multiple sites on one chromosome (n = 1). We also show that small ratio changes (Δ log2ratio ∼ 0.1) distinguish homozygous and heterozygous regions of the genome in interspecific backcross mice, providing an efficient method for genotyping progeny of backcrosses.

Documentos Relacionados

• Detecting single DNA copy number variations in complex genomes using one nanogram of starting DNA and BAC-array CGH
• A statistical approach for array CGH data analysis
• Whole Genome Analysis of Genetic Alterations in Small DNA Samples Using Hyperbranched Strand Displacement Amplification and Array–CGH
• Array-CGH testing in spontaneous abortions with normal karyotypes
• Detection and Characterization of NF1 Microdeletions by Custom High Resolution Array CGH