Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.
AUTOR(ES)
Lewis, J B
RESUMO
Cytoplasmic RNA, isolated from cells late after infection by adenovirus type 2 and fractionated by hybridization to specific fragments of adenovirus DNA produced by cleavage with the endonuclease R-EcoRI, was used as template for protein synthesis in cell-free mammalian extracts. Each of the R-EcoRI fragments of DNA selects RNA that encodes specific subsets of the viral polypeptides. From the known order of the R-EcoRI fragments, the following partial map is deduced: (III, IIIa, IVa2, V, P-VII, IX), (II, P-VI), 100K, IV-where the relative order of the components enclosed in parentheses has not yet been determined.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=432530Documentos Relacionados
- Cell-free protein synthesis directed by Q beta-RNA and by two specific fragments.
- Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
- Mapping of the vaccinia virus DNA polymerase gene by marker rescue and cell-free translation of selected RNA.
- Cell-Free Translation of Highly Purified Adenovirus Messenger RNA
- Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.