Mapeamento do potencial biossintético em linhagens de Streptomyces / Mapping the biosynthetic potential in Streptomyces strains

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

26/07/2011

RESUMO

Actinobacteria are important sources of new molecules with biological activities. Streptomyces are the most important genera studied. Such microorganisms carries out among a variety of biosynthetic enzimes, two main groups known as polyketide synthase (PKS) and non ribosomal peptide synthetase (NRPS), both catalyzing the biosynthesis of polyketide and non ribosomal peptides respectively. PKS and NRPS biosynthesis follows a collinear relationship among the gene cluster, the enzyme and the secondary metabolite. Therefore, the knowledge of the gene sequences allows to find new molecules. In this work we adopted a strategy to map the biosynthetic genes without the need of extensive whole genome sequencing in order to predict the metabolite produced by Streptomyces strains isolated from Citrus ssp. The strains were also cultivated and the metabolite production monitored by liquid chromatography coupled with mass spectrometry. In addition, the biological activity of these medium was estimated using qualitative biochemical assays.

ASSUNTO(S)

streptomyces genome mining peptídeo não ribossomal streptomyces genome mining polyketide nonribosoma peptide policetideo

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