Manipulation of a quasi-natural cell block for high-efficiency transplantation of adherent somatic cells
AUTOR(ES)
Chung, H.J., Hassan, M.M., Park, J.O., Kim, H.J., Hong, S.T.
FONTE
Braz J Med Biol Res
DATA DE PUBLICAÇÃO
03/03/2015
RESUMO
Recent advances have raised hope that transplantation of adherent somatic cells could provide dramatic new therapies for various diseases. However, current methods for transplanting adherent somatic cells are not efficient enough for therapeutic applications. Here, we report the development of a novel method to generate quasi-natural cell blocks for high-efficiency transplantation of adherent somatic cells. The blocks were created by providing a unique environment in which cultured cells generated their own extracellular matrix. Initially, stromal cells isolated from mice were expanded in vitro in liquid cell culture medium followed by transferring the cells into a hydrogel shell. After incubation for 1 day with mechanical agitation, the encapsulated cell mass was perforated with a thin needle and then incubated for an additional 6 days to form a quasi-natural cell block. Allograft transplantation of the cell block into C57BL/6 mice resulted in perfect adaptation of the allograft and complete integration into the tissue of the recipient. This method could be widely applied for repairing damaged cells or tissues, stem cell transplantation, ex vivo gene therapy, or plastic surgery.
Documentos Relacionados
- Cyclic amphipathic peptide-DNA complexes mediate high-efficiency transfection of adherent mammalian cells.
- Large-scale, high-efficiency production of coffee somatic embryos
- High-efficiency transformation of bacterial cells by electroporation.
- Engineering of Quasi-Natural Pseudomonas putida Strains for Toluene Metabolism through an ortho-Cleavage Degradation Pathway
- High-efficiency transformation of mammalian cells by plasmid DNA.