MANIFESTATIONS OF INJURY IN YEAST CELLS EXPOSED TO SUBZERO TEMPERATURES I. : Morphological Changes in Freeze-Substituted and in “Frozen-Thawed” Cells
AUTOR(ES)
Mazur, Peter
RESUMO
Mazur, Peter (Oak Ridge National Laboratory, Oak Ridge, Tenn.). Manifestations of injury in yeast cells exposed to subzero temperatures. I. Morphological changes in freeze-substituted and in “frozen-thawed” cells. J. Bacteriol. 82:662–672. 1961.—When cells of the yeast Saccharomyces cerevisiae are cooled rapidly to −30 C or below, fewer than 0.01% survive. In contrast, when they are cooled slowly, up to 50% survive. The effect of cooling rate on survival was reflected in the morphological appearance of cells both before and after thawing. Appearance before thawing was observed by fixing the cells at subzero temperatures by freeze-substitution with cold ethanol. Slowly cooled freeze-substituted cells were considerably smaller and more flattened than those cooled rapidly. The differences in appearance and the differences in survival are both consistent with the view that intracellular ice formation occurs more extensively in rapidly cooled cells and is responsible for their higher mortality.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=279233Documentos Relacionados
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