Magnetic techniques for the isolation and purification of proteins and peptides

AUTOR(ES)

Safarik, Ivo

FONTE

BioMed Central

RESUMO

Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques.

Documentos Relacionados

• Isolation and Purification of the Envelope Proteins of Newcastle Disease Virus
• Magnetic DNA affinity purification of yeast transcription factor tau--a new purification principle for the ultrarapid isolation of near homogeneous factor.
• Use of the same polymer for synthesis and purification of peptides
• Comparison and Combination of Techniques for Determining the Parameters of a Magnetic Hysteresis Model
• Method for Isolation and Purification of Cyanobacteria