Lysis of Staphylococcus aureus Cell Walls by a Soluble Staphylococcal Enzyme

AUTOR(ES)

Huff, Eskin

RESUMO

Enzyme preparations of Staphylococcus aureus were examined for their ability to solubilize 32P-labeled cell walls of the parent organism. Enzymatic activity was observed in the growth medium, in soluble fractions, and associated with native cell walls. Enzyme associated with isolated cell walls could be inactivated with formaldehyde without reducing the susceptibility of the walls to the action of added enzyme. When cells are frozen and thawed, 50 to 75% of the intracellular enzyme is released along with 2% of the intracellular protein. This freeze-thaw extracted enzyme has little, if any, activity on intact S. aureus cells. It appears that the enzyme resides near the cell wall and acts on the cell-wall inner surface.

Documentos Relacionados

• Electron Microscopy of the Lysis of Staphylococcus aureus Cell Walls by Aeromonas Lytic Factor
• Lysis of Bacterial Cell Walls by an Enzyme Isolated from a Myxobacter
• Relative Rates of Lysis of Staphylococcal Cell Walls by Lytic Enzymes from Various Bacteriophage Types
• Electron Microscopy of Staphylococcus aureus Cell Wall Lysis
• Enzyme-linked immunosorbent assay for detection of antibodies to Staphylococcus aureus cell walls in experimental osteomyelitis.