Lysine Biosynthesis in Barley (Hordeum vulgare L.) 1
AUTOR(ES)
Møller, Birger Lindberg
RESUMO
Lysine biosynthesis in seedlings of barley (Hordeum vulgare L. var. Emir) was studied by direct injection of the following precursors into the endosperm of the seedlings: acetate-1-14C; acetate-2-14C; pyruvate-1-14C; pyruvate-2-14C; pyruvate-3-14C; alanine-1-14C; aspartic acid-1-14C; aspartic acid-2-14C; aspartic acid-3-14C; aspartic acid-4-14C; α-aminoadipic acid-1-14C; and α, ε-diaminopimelic acid-1-(7)-14C. The distribution of activity in the individual carbon atoms of lysine in the different biosynthetic experiments was determined by chemical degradation. The incorporation percentages and labeling patterns obtained are in agreement with the occurrence of the diaminopimelic acid pathway. The results do not fit the incorporation percentages and labeling patterns expected if the α-aminoadipic acid pathway was operating. However, the results show that barley seedlings are able to convert a small part of the α-aminoadipic acid administered directly to lysine.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=367467Documentos Relacionados
- Lysine Catabolism in Barley (Hordeum vulgare L.)
- Phosphoinositides in Barley (Hordeum vulgare L.) Aleurone Tissue.
- Inositol Trisphosphate Metabolism in Subcellular Fractions of Barley (Hordeum vulgare L.) Mesophyll Cells.
- AFLP marker linked to water-stress-tolerant bulks in barley (Hordeum vulgare L.)
- Properties and Regulation of Aspartate Kinase from Barley Seedlings (Hordeum vulgare L.)
