Lymphocyte Transformation during Dinitrochlorobenzene Contact Sensitization. AN IN VITRO AND IN VIVO EVALUATION OF THE PRIMARY IMMUNE RESPONSE IN MAN

AUTOR(ES)

Miller, A. Edgar

RESUMO

An evaluation of cell-mediated immunity in man is described that combines the advantages of an in vitro technique, lymphocyte transformation, with the use of contact sensitization to a primary immunogen, dinitrochlorobenzene (DNCB). DNCB, when coupled to autologous or allogeneic peripheral blood leukocytes, forms a complex, DNCB-antigen, that induces lymphocyte transformation specifically in leukocyte cultures from subjects sensitized to DNCB. Sequential studies of lymphocyte transformation to DNCB-antigen show that specifically reactive lymphocytes are first detected at about 10 days after in vivo application of a sensitizing dose of DNCB and reach a peak at about 14-21 days.

Documentos Relacionados

• Lymphocyte Transformation in Syphilis: an In Vitro Correlate of Immune Suppression In Vivo?
• Streptomycin sensitization.
• Suppression of In Vitro Lymphocyte Transformation During an Experimental Dermatophyte Infection
• Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E: IN VITRO LYMPHOCYTE TRANSFORMATION AND ELABORATION OF LYMPHOCYTE MEDIATORS
• An Evaluation of the Normal Lymphocyte Transfer Test in Man*